On June 21-22, the United States and Iran held their first round of talks since signing a ceasefire-centered memorandum of understanding on June 17, The New York Times reported on June 22. U.S. Vice President JD Vance said negotiators made progress toward establishing a bilateral line of communication to facilitate maritime transits through the Strait of Hormuz, creating a deconfliction cell to de-escalate the Israel-Hezbollah conflict in Lebanon, and resuming nuclear inspections by the International Atomic Energy Agency in Iran.

After the first round of negotiations concluded on June 22, the U.S. Treasury Department issued a 60-day sanctions waiver permitting the sale of Iranian oil. The negotiations occurred after an initial postponement on June 19, likely due in part to Israeli military operations against Hezbollah in Lebanon. Iran's negotiators then threatened to walk out of the negotiations after Trump threatened to attack Iran again on June 21. Iran's Islamic Revolutionary Guard Corps said the Strait of Hormuz was closed on June 20 due to ceasefire violations, including Israeli military operations against Hezbollah, though U.S. Central Command denied the IRGC's claim. Separately, there was an explosion late on June 21 at Qatar's Ras Laffan export terminal as workers tried to resume operations, resulting in at least 13 fatalities and several more injuries.